Bookmark and Share

Basic Techniques in Immunology


Technical University of Denmark


General course objectives:
To learn how to operate machinery and equipment relevant in the field of Immunology and how to design and execute experiments using this machinery.

Learning objectives:
A student who has met the objectives of the course will be able to:
  • operate flow cytometers.
  • operate fluorescent and confocal microscopes.
  • operate 10X Chromium and BD Rhapsody for single-cell RNA-sequencing experiments.
  • sort immune cells using flow cytometry.
  • operate basic equipment used in biochemistry (e.g. electrophoresis, PCR, Western blot).
  • design experiments using the above equipment
  • work under sterile conditions in a cell lab.
  • safely work with biological material and chemicals.
  • design and plan an experimental protocol selecting appropriate techniques.

Contents:
The course is relevant to all new PhD students in immunology. They will learn how to operate basic equipment used to conduct immunological experiments. These include flow cytometers (for analysis and sorting), microscopes, 10X Chromium and BD Phapsody as well as basic equipment used in most biochemical applications (e.g. PCR and electrophoresis). The students will also learn how to work under sterile conditions in a cell lab and how to safely work with biological material and various chemicals. Moreover, students will receive training in how to design relevant experiments (e.g. construct antibody panels in flow cytometry). Teaching time will be determined between the student and the teacher of the relevant subject. Relevant teaching material will be supplied by the teacher in advance. The teacher will be responsible for ensuring that the student receives sufficient training to be able to operate the above machinery independently and without supervision.

Back
Course organizer
Vasileios , Sebastien , Mohammad , Marlene Danner
Place/Venue
Anker Engelunds Vej 1
City
2800 Kgs. Lyngby
Country
Denmark
Workload
5
Link
http://kurser.dtu.dk/course/22211