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Advanced Synthetic Biology for Creating Sustainable Cell Factories


Technical University of Denmark


General course objectives:
To provide the student with extensive theoretical knowledge and practical experience on state-of-the-art synthetic biology, computational biology and metabolic engineering for development of industrial cell factories for sustainable production of chemicals. Students will learn about all phases of cell factory design and engineering. This includes topics ranging from life cycle assessment and techno-economic analysis to metabolic engineering and the scaling up of bioprocesses.

Learning objectives:
A student who has met the objectives of the course will be able to:
  • Select a candidate host strain, carbon feedstock, and fermentation mode for a given bioproduct based on preliminary techno-economic analysis.
  • Reconstruct a biosynthetic pathway towards a given bioproduct using computational tools and databases.
  • Conceptually design a strain development program.
  • Optimization and adaptation of enzymes for their application in cell factories.
  • Design in silico specific genetic modifications for five typical hosts: E. coli, S. cerevisiae, P. putida & Streptomyces, based on state-of-the-art synthetic biology tools.
  • Understand challenges associated with up-scaling bioproduction.
  • Apply avdvanced synthetic biology tools to engineer one of the four hosts in the laboratory.
  • Comply with Good Laboratory Practice (GLP): register the data in Laboratory Information Management System (LIMS) and maintain electronic laboratory notebooks.
  • Analyze and interpret experimental results.
  • Present the performed experimental study for an audience.

Contents:
The students will learn how to apply the state-of-the-art synthetic biology tools for designing novel cell factories for production of bio-based chemicals and pharmaceuticals. The course is composed of theory lectures and computer exercises in the morning and laboratory project work in the afternoon. The students will choose one of the four experimental tracks, where they will carry out a smaller research project on engineering of: (1) E. coli, (2) S. cerevisiae, (3) Streptomyces, or (4) Pseudomonas putida.

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Course organizer
Pablo Ivan , Christoph Daniel , Emil Damgaard
Place/Venue
Anker Engelunds Vej 1
City
2800 Kgs. Lyngby
Country
Denmark
Workload
5
Link
http://kurser.dtu.dk/course/29902