NMR Spectroscopy
Tampere University
Core content
· Physical basis of NMR spectroscopy.
· Instrumentation and principles of data acquisition and processing.
· Experimental setup for 1H and 13C spectra by pulsed methods.
· Interpretation of the first-order 1H and 13C spectra based on chemical shifts and coupling constants. Determination of the structure of a simple unknown compound.
· Interpretation of APT/DEPT 13C spectra and 2D homo- and hetero-correlated spectra (H,H-COSY, H,C-HSQC, NOESY).
· Practical skills in setup and measurement of 1H and 13C NMR-spectra. and 2D spectra
Complementary knowledge
· Determination of relaxation times.
· Decoupling principles, Nuclear Overhauser effect.
· Interpretation of higher order 1H and 13C spectra and determination of the structure of more complex unknown compounds.
· Complex 1D pulse sequences, COSY pulse sequence.
Specialist knowledge
· Interpretation of higher order 1H and 13C spectra and determination of the structure of more complex unknown compounds.
· Polarization transfer, INEPT and HETCOR, indirect detection experiments HSQC HMBC
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